Thymidine mutants

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Mutants affecting thymidine metabolism in Neurospora crassa.

When (14)C-thymidine labeled only in the ring is administered to Neurospora crassa, the majority of the recovered label is found in the ribonucleic acid (RNA). Three mutants were isolated in which different steps are blocked in the pathway that converts the pyrimidine ring of thymidine to an RNA precursor. Evidence from genetic, nutritional, and accumulation studies with the three mutants shows...

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Genetic and biochemical properties of thymidine-dependent mutants of pneumococcus.

Independently isolated thymidine-dependent strains were shown to be defective in thymidylate synthetase and mutated at separable but closely linked sites.

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Inhibition of thymidine transport in dnaA mutants of Escherichia coli.

DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli. We report here our evidence that thymidine transport across cytoplasmic membranes in temperature-sensitive dnaA mutants is greatly decreased at a permissive temperature for growth of the mutants. Complementation analysis with a plasmid containing the wild type dnaA gene and P1 phage-mediated transduction confirmed...

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Diverse herpes simplex virus type 1 thymidine kinase mutants in individual human neurons and Ganglia.

Mutations in the thymidine kinase gene (tk) of herpes simplex virus type 1 (HSV-1) explain most cases of virus resistance to acyclovir (ACV) treatment. Mucocutaneous lesions of patients with ACV resistance contain mixed populations of tk mutant and wild-type virus. However, it is unknown whether human ganglia also contain mixed populations since the replication of HSV tk mutants in animal neuro...

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Thymidine Kinases from Human and Caenorhabditis elegans and their Active Site Mutants

Thymidine kinase 1 (TK1) is a deoxynucleoside kinase that catalyzes the transfer of γ-phosphate from ATP to the 5`-OH of thymidine (dThd) to form thymidine monophosphate. In humans and other mammalians TK1 is a key enzyme in the salvage of dTTP for DNA synthesis. Deoxynucleoside kinases have several useful applications, but one of the more important ones is the activation of antiviral and antic...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1978

ISSN: 0021-9746

DOI: 10.1136/jcp.31.8.808-b